BirdnestS22.pptx

Nest predation: Developing a question and purpose:

Example of a scientific question: Does nest height matter for influencing nest predation?

Remember developing a question(hypothesis) for scientific research must meet the following requirements:

Testable

Repeatable

Measurable; through statistical analysis

Should be influenced by the scientific literature

Should have a reason for testing; i.e., Why do we care, or why is it important to society, the environment, the public, etc.

Tentative Location: (M,W, F classes)

Flat Rock State Natural Area

2381 Factory Road,

Murfreesboro, TN 37130

860 17’ 43”W, 350 51’ 30”N

Analyzing Ecology:

Independent variables: factors that are presumed to cause other variables to change.

Dependent variables: factors that are being changed.

Example:

We hypothesize that variation in the nest condition has led to different predation rates.

Independent variable = Nest condition

Dependent variable = Predation

* Experimental unit = nest

5.5ft

Use transects and marking them

Map it or sketch it

Assign treatment/control conditions along transects

Random table generator or random # table

Pattern

Random

Combination

Layout experiment

Brown Egg Nest

Speckled Egg Nest

POSITIVE SIGNS OF PREDATION

For this class we focus on Chi Squared Statistical Method:

“Goodness of Fit” Test

2X2 Contingency Table analysis

It is intended to test how likely it is that an observed distribution is due to chance. It is also called a "goodness of fit" statistic, because it measures how well the observed distribution of data fits with the distribution that is expected if the variables are independent.

DataHigh NestLow nestTotal
Predation5611
No Predation111021
Total161632

Data Collection:

Dependent Variable 

Independent Variable 

Pred/NoTrt.Obs.Calc.Calc.Expected
Predhigh5Col1 x row1/Total(16 x 11)/32 5.5
Predlow6Col2 x row1/total(16 x 11)/32 5.5
No Predhigh11Col1 x row2/total(16 x 21)/32 10.5
No Predlow10Col2 x row2/total(16 x 21)/32 10.5

How to calculate expected frequencies:

DataHigh NestLow nestTotal
Predation5611
No Predation111021
Total161632
Pred/NoTrt.Obs.Exp.(Obs – Exp)2 /Exp
PredHigh5 5.5(5 – 5.5)2/ 5.50.045
PredLow6 5.5(6 – 5.5)2/5.50.045
No PredHigh11 10.5(11 – 10.5)2/10.50.024
No PredLow10 10.5(10 – 10.5)2/10.50.024
Sum of Chi-square0.139

Calculating Chi-square:

From the observed and expected frequencies

x2 = Σ(observed – expected)2

expected

x2 = (5-5.5) 2+ (11-10.5) 2+ (6-5.5) 2+ (10-10.5) 2

5.5 10.5 5.5 10.5

x2 = 0.139

Hypothesis – Additional info and X2(Chi square):

H0: The height of the nest has no effect on predation of eggs

HA: The height of the nest does have an effect on predation of eggs

Each group will have a X2(chi square critical value of 3.841), so if the calculated test statistic is…..

≥ critical value means that it supports the hypothesis of a difference (reject Ho)

≤ critical value means that it does NOT support the hypothesis of a difference (accept Ho))

Interpretation

Because our x2 is less than the critical value for p = 0.05, we fail to reject the null hypothesis

There is no significant difference in the predation of nests placed high versus nests that low

X2 = 0.139

critical value (from table, p = 0.05) = 3.84

0.139 < 3.84

13

Your Project:

Model birds find at Flat Rock

Lists on D2L

Control & treatment

Manipulate Nest or egg

Shape, size, color, materials, or decoy